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fluorescent protein gfp_loxp_genscript  (GenScript corporation)

 
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    Structured Review

    GenScript corporation fluorescent protein gfp_loxp_genscript
    Dual-marker cassettes facilitate tagging of genes with <t>fluorescent</t> protein transgenes or other epitopes. (A) Schematic of a dual-marker cassette his-72 :: GFP tagging vector. The selection cassette is housed within a constitutively spliced synthetic intron (dotted lines denote 5′ and 3′splice sites), such that after Cre -mediated excision of the cassette, the remaining loxP site will be spliced out of the mature GFP mRNA open reading frame, creating near seamless editing. (B) Fluorescent micrographs of endogenously tagged HIS-72::GFP (top) and HIS-72::mCherry (bottom). Scale bars, 100 µm.
    Fluorescent Protein Gfp Loxp Genscript, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent protein gfp_loxp_genscript/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    fluorescent protein gfp_loxp_genscript - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes"

    Article Title: Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes

    Journal: Genetics

    doi: 10.1534/genetics.115.180679

    Dual-marker cassettes facilitate tagging of genes with fluorescent protein transgenes or other epitopes. (A) Schematic of a dual-marker cassette his-72 :: GFP tagging vector. The selection cassette is housed within a constitutively spliced synthetic intron (dotted lines denote 5′ and 3′splice sites), such that after Cre -mediated excision of the cassette, the remaining loxP site will be spliced out of the mature GFP mRNA open reading frame, creating near seamless editing. (B) Fluorescent micrographs of endogenously tagged HIS-72::GFP (top) and HIS-72::mCherry (bottom). Scale bars, 100 µm.
    Figure Legend Snippet: Dual-marker cassettes facilitate tagging of genes with fluorescent protein transgenes or other epitopes. (A) Schematic of a dual-marker cassette his-72 :: GFP tagging vector. The selection cassette is housed within a constitutively spliced synthetic intron (dotted lines denote 5′ and 3′splice sites), such that after Cre -mediated excision of the cassette, the remaining loxP site will be spliced out of the mature GFP mRNA open reading frame, creating near seamless editing. (B) Fluorescent micrographs of endogenously tagged HIS-72::GFP (top) and HIS-72::mCherry (bottom). Scale bars, 100 µm.

    Techniques Used: Marker, Plasmid Preparation, Selection



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